What is the confirmatory test for glucose?
a) Fehling’s Test: Fehling’s Solution (deep blue colored) is used to determine the presence of reducing sugars and aldehydes. Perform this test with fructose, glucose, maltose and sucrose.
What is the confirmatory test for aniline?
azo dye test
Aromatic primary amines can be confirmed by azo dye test. Primary amine e.g. aniline reacts with nitrous acid generated in situ by the reaction of sodium nitrite with HCl at 0–5°C to produce diazonium salt. This couples with β-naphthol to give a scarlet red dye, which is sparingly soluble in water.
What tests detect proteins?
the Biuret test for
The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.
What is anthrone reagent test?
Uses of Anthrone Test-Anthrone test is used for the detection and quantification of carbohydrates in various samples like blood serum, milk, and its variation, etc.
What is amine test?
A for BACTERIAL VAGINOSIS-vaginal infection with Gardnerella vaginalis -sometimes called the ‘fish’ test. A drop of vaginal discharge is mixed with a drop of saline on a slide and a drop of 5% potassium hydroxide added. If positive, a fishy amine smell is at once apparent.
What is the colour of anthrone reagent?
reagent is anthranol, the enol tautomer of anthrone, which reacts by condensing with the carbohydrate furfural derivative to give a green colour in dilute and a blue colour in concentrated solutions, which is determined colorimetrically.
What is Kabil Mi reaction?
The carbylamine reaction (also known as the Hoffmann isocyanide synthesis) is the synthesis of an isocyanide by the reaction of a primary amine, chloroform, and base. The conversion involves the intermediacy of dichlorocarbene.
What is isocyanide test 12?
A test for primary amines by reaction with an alcoholic solution of potassium hydroxide and trichloromethane. RNH2+3KOH+CHCl3 → RNC+3KCl+3H2O The isocyanide RNC is recognized by its unpleasant smell. This reaction of primary amines is called the carbylamine reaction.
What is orange dye test?
Answer: This test is performed in order to confirm the presence of aromatic amines. Aromatic amines forms a diazonium salts when treated with HNO2. These diazo compounds couple with phenols like β-naphthols forms an orange azo dye which is insoluble in water.
What is azo dye used for?
Azo dyes are the most important synthetic colorants which have been widely used in textile, printing, paper manufacturing, etc.